cDNA Microarray Basics
cDNA Microarray Imaging
cDNA Image Noise
cDNA Characteristics
cDNA Image Denoising
cDNA Data Normalization
cDNA Spot Localization
cDNA Spot Segmentation


cDNA Spot Localization

cDNA spot localization solutions use the cDNA microarray image as the input and transforms it into an edge map which can be viewed as a line drawing image with a spatial resolution identical to that of the input. An intensity edge map corresponds to sudden changes in image characteristics which tend to occur at spot boundaries and the locations of these changes reflect the shapes of cDNA spots.

Acquired cDNA image


Localized spots


Multichannel images such as cDNA microarray images carry information contained in the various spectral channels. This information can be used to identify and detect spot edges. The boundary between two surfaces with different properties can be determined in more than one way, as the edge formation can be attributed to changes in one channel or in both channels simultaneously. Since cDNA images can be viewed as vector fields, edges can potentially be  detected as differences in local vector statistics which accurately reflect variations in spectral information and spatial image content. Moreover, due to the utilization of the robust order-statistic principle, the nonlinear vector operators can be less sensitive to noise compared to the conventional edge operators such as Sobel, Prewitt, and Canny edge detectors.


bulletR. Lukac and K.N. Plataniotis: A Taxonomy of Color Image Filtering and Enhancement Solutions. In Advances in Imaging and Electron Physics, (ed.) P.W. Hawkes, Elsevier/Academic Press, vol. 140, pp.187-264, June 2006.
bulletR. Lukac, B. Smolka, K. Martin, K.N. Plataniotis, and A.N. Venetsanopoulos, "Vector Filtering for Color Imaging," IEEE Signal Processing Magazine, Special Issue on Color Image Processing, vol. 22, no. 1, pp. 74-86, January 2005.
bulletR. Lukac, K.N. Plataniotis, B. Smolka, and A.N. Venetsanopoulos, "A Multichannel Order-Statistic Technique for cDNA Microarray Image Processing," IEEE Transactions on NanoBioscience, vol. 3, no. 4, pp. 272-285, December 2004.

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Last update: 10/15/06

2006 Rastislav Lukac