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cDNA Microarray Basics
cDNA Microarray Imaging
cDNA Image Noise
cDNA Characteristics
cDNA Image Denoising
cDNA Data Normalization
cDNA Spot Localization
cDNA Spot Segmentation

 

cDNA Microarray Characteristics

To effectively process microarray images, the differences among cDNA vectors spawned by the supporting window of the image processing solution have to be quantified. Since each cDNA vector is uniquely determined by its magnitude and direction in the two-component RG space, the microarray image processing operators performing data normalization, image denoising and enhancement, and spot localization and segmentation should take into consideration both the magnitude and the orientation of the cDNA samples.

 


Ideal cDNA image

Under the ideal conditions, each spot has its own unique magnitude and directional characteristics. Unfortunately, due to the nature of cDNA microarray technology the ideal noise free-data are unavailable in cDNA microarray imaging, and the acquired cDNA vectors differ in both the magnitude and the direction.


Real cDNA image

 
 


Magnitude characteristics of the ideal cDNA image

 


Magnitude characteristics of the real cDNA image

 
 


Directional characteristics of the ideal cDNA image

 


Directional characteristics of the real cDNA image

 

References:

 
bulletR. Lukac and K.N. Plataniotis, "cDNA Microarray Image Segmentation Using Root Signals,"  International Journal of Imaging Systems and Technology, vol. 16, no. 2, pp. 51-64, April 2006.
bulletR. Lukac, K.N. Plataniotis, B. Smolka, and A.N. Venetsanopoulos, "cDNA Microarray Image Processing Using Fuzzy Vector Filtering Framework," Fuzzy Sets and Systems, Special Issue on Fuzzy Sets and Systems in Bioinformatics, vol. 152, no. 1, pp.17-35, May 2005.
bulletR. Lukac, B. Smolka, K. Martin, K.N. Plataniotis, and A.N. Venetsanopoulos, "Vector Filtering for Color Imaging," IEEE Signal Processing Magazine, Special Issue on Color Image Processing, vol. 22, no. 1, pp. 74-86, January 2005.
bulletR. Lukac, K.N. Plataniotis, B. Smolka, and A.N. Venetsanopoulos, "A Multichannel Order-Statistic Technique for cDNA Microarray Image Processing," IEEE Transactions on NanoBioscience, vol. 3, no. 4, pp. 272-285, December 2004.

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Last update: 10/15/06

2006 Rastislav Lukac